DNA extraction and purification
Research genomic DNA, plasmid or bacmid DNA, viral DNA, and DNA cleanup workflows with attention to integrity, inhibitor removal, yield, and downstream amplification or cloning.
San Diego, California
DNA and RNA purification services for research samples
VexisBio provides sample-specific nucleic acid extraction, purification, cleanup, concentration, and quality-control support. Each project begins with the sample type and downstream application so the resulting DNA or RNA is prepared for PCR, qPCR, RT-qPCR, sequencing, cloning, transfection, or another agreed research use.
Nucleic acid purification
Match the extraction method to the sample and downstream assay
DNA and RNA quality depends on more than total yield. Sample composition, nuclease exposure, inhibitors, fragment size, cellular or extracellular origin, and the next analytical step determine the appropriate workflow.
RNA extraction and purification
Total RNA or target-appropriate RNA workflows designed to limit degradation and prepare material for RT-qPCR, sequencing, expression analysis, or other research applications.
Nucleic acid cleanup
Concentration, buffer exchange, and removal of proteins, salts, enzymes, phenol, guanidinium, or other contaminants from existing DNA or RNA preparations.
Sample types and downstream uses
Acceptance depends on sample state, quantity, biosafety classification, shipping conditions, and requested output. Contact VexisBio before shipping biological material.
Biological samples
- Cultured mammalian or insect cells
- Microbial cell pellets
- Research tissues or homogenates
- Serum, plasma, or cell-free fractions
- Other approved research matrices
Plasmid, bacmid, and viral material
- Plasmid-bearing bacterial cultures
- Large bacmid DNA preparations
- Clarified research viral material
- DNase-resistant viral genome workflows
- Existing extracts requiring cleanup
Downstream preparation
- PCR, qPCR, or RT-qPCR
- Sanger or next-generation sequencing
- Cloning or restriction analysis
- Transfection-quality planning
- Research assay development
Purification and quality-control options
The chemistry and QC panel are selected for the project. Method choice may include membrane, magnetic-bead, precipitation, organic-extraction, or other sample-appropriate workflows.
Extraction and cleanup
- Sample-specific lysis and nuclease control
- DNA/RNA binding and washing
- Protein and inhibitor removal
- Optional DNase or RNase treatment
- Concentration and buffer exchange
Quantity and purity
- Absorbance concentration and purity ratios
- Fluorometric DNA or RNA quantification
- Yield and recovery documentation
- Final volume and concentration adjustment
- Aliquoting and storage planning
Fitness for use
- Gel or electrophoretic integrity assessment
- Amplification-suitability checks
- Cloning or restriction suitability when agreed
- Sequencing-input planning
- Method and QC summary
From sample review to qualified DNA or RNA
| Stage | Typical work | Output |
|---|---|---|
| Sample and use review | Define sample, storage, biosafety, target nucleic acid, downstream assay, and acceptance needs. | Recommended workflow and sample requirements. |
| Pilot or method selection | Evaluate lysis, purification, recovery, and inhibitor control where needed. | Pilot results and selected method. |
| Sample processing | Process agreed samples with research-scope contamination controls and sample tracking. | Purified DNA or RNA. |
| QC and delivery | Perform contracted concentration, purity, integrity, or fitness-for-use checks. | Research material, QC results, and handling guidance. |
Information needed for a quote
- Sample type, species, and biosafety information
- Number of samples and approximate input
- Storage buffer and temperature
- Target nucleic acid and final format
- Downstream assay or application
- Required quality, yield, and timeline
Do not ship biological samples until acceptance and shipping instructions are confirmed.
Typical deliverables
- Purified research-use DNA or RNA
- Sample manifest and processing summary
- Concentration and agreed purity results
- Integrity or amplification results when included
- Final buffer, volume, and storage information
- Technical limitations and next-step recommendation
Clinical testing, diagnostic reporting, and regulated chain-of-custody services are not implied unless separately reviewed and contracted.
Discuss your DNA or RNA purification project
Send the sample type, number of samples, target nucleic acid, downstream application, quality requirements, and timeline for an initial scope review.
Request a Purification Quote