ELISA-qPCR workflows for high-sensitivity biomarker assays

VexisBio supports immuno-qPCR style assay planning where protein recognition, antibody-oligo conjugation, qPCR readout, and assay credibility must work together.

ELISA-qPCR antibody oligo conjugation

Conjugation strategy

Antibody-oligo format selection, chemistry considerations, purification approach, and reagent compatibility planning.

Assay workflow

Capture/detection design, background control, qPCR readout considerations, controls, and sensitivity planning.

Translational framing

Clear explanation of where ELISA-qPCR adds value compared with conventional ELISA or standard qPCR-only workflows.

Best-fit programs

Low-abundance protein biomarkers, exploratory high-sensitivity workflows, antibody-oligo reagent development, and assay formats that need both immuno-recognition and nucleic-acid amplification logic.

Start with

  • Target and expected concentration
  • Available antibody pair
  • Sample matrix
  • Desired readout and equipment
  • Current protocol or feasibility data