Description
Product Details
Item Number: SN-NfL-ELISA-96
Brand: SynerviaTM
Human NF-L ELISA Kit (RUO)
Quantify Neurofilament Light (NF-L) Antigen with High Sensitivity & Accuracy
Product Overview
The SynerviaTM NF-L Antigen ELISA Kit is designed for the quantification of human NF-L antigen in serum, plasma, or biopsy extractions. This research-use-only (RUO) kit offers high sensitivity, specificity, and reproducibility, making it ideal for neurodegenerative disease research.
- Catalog No.: E20142019
- Format: 96-well Plate (12 x 8 strips)
- Detection Range: 10 pg/mL – 1500 pg/mL
- Minimum Detectable Concentration: 8.5 pg/mL
Principle of the Test
This sandwich ELISA utilizes a capture antibody pre-coated onto a 96-well plate. The NF-L antigen in the sample binds to the capture antibody, followed by detection with an HRP-conjugated antibody. A substrate reaction produces a color change, which is quantified by measuring absorbance at 450 nm.
Kit Contents
| Component | Description |
|---|---|
| Pre-coated ELISA Plate | 96-well plate with immobilized anti-NF-L antibody |
| NF-L Standards | 6 concentrations (10 – 1500 pg/mL) |
| Positive & Negative Controls | Included |
| qEIAmix Detection Mix | HRP-polymer conjugated antibody |
| Substrate Solution | Colorimetric detection reagent |
| Wash Buffer (10X) | For plate washing |
| Assay Diluent & Stop Solution | Included |
Sample Requirements
- Serum: Collect in a serum separator tube, allow clotting, and centrifuge at 1,000 × g for 10 minutes.
- Plasma: Collect in citrate, heparin, or EDTA tubes, centrifuge at 1,000 × g for 10 minutes.
- Tissue Extraction: Follow appropriate tissue preparation protocols.
*All samples should be stored at < -20°C to prevent degradation.*
Performance Characteristics
- Sensitivity: 8.5 pg/mL
- Specificity: No observed cross-reactivity
- Precision: Intra-assay CV <10%, Inter-assay CV <15%.
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