Description
Product Details
Item Number: E21231218
Brand: SynerviaTM
HE4 Antigen ELISA Kit
High-Sensitivity Quantification of HE4 in Biological Samples
Product Overview
The SynerviaTM HE4 Antigen ELISA Kit is designed for the quantification of recombinant or native HE4 protein in **serum, plasma, and cell culture supernatant**. This highly sensitive and specific assay is ideal for **ovarian cancer research and biomarker studies**.
- Catalog No.: E21231218
- Format: 96-well Plate (12 x 8 strips)
- Detection Range: 2.73 pM – 21 pM
- Sensitivity: 1 pM
Principle of the Test
This **sandwich ELISA** utilizes a **capture antibody** pre-coated onto a 96-well plate. The HE4 antigen in the sample binds to the capture antibody, followed by detection with a **biotinylated antibody and Avidin-HRP**. A colorimetric reaction is used for detection, and absorbance is read at **450 nm**.
Kit Contents
| Component | Description |
|---|---|
| Pre-coated ELISA Plate | 96-well plate with immobilized anti-HE4 antibody |
| HE4 Standards | 6 concentrations (0 – 21 pM) |
| Biotin-Enzyme Mix A & B | For antigen detection |
| Wash Buffer (10X) | 25 mL |
| Substrate & Stop Solution | 10 mL each |
Sample Requirements
- Serum: Collect, allow clotting, and centrifuge at 1,000 × g for 10 minutes.
- Plasma: Collect in citrate, heparin, or EDTA tubes, centrifuge at 1,000 × g for 10 minutes.
- Cell Culture Supernatant: Centrifuge to remove debris before analysis.
Performance Characteristics
- Sensitivity: 1 pM
- Specificity: High selectivity for HE4
- Precision: Intra-assay CV <10%, Inter-assay CV <15%.
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