Description

Product Specification

Species: Human

Synonyms: Tau-F, Tau-4, 2N4R, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), MAPT, MAPTL, MTBT1, TAU

Accession: P10636-8

Amino Acid Sequence, Protein sequence (P10636-8, Met1-Leu441, with C-His tag) MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Expression System: CHO

Molecular Weight: Predicted MW: 47.5 kDa Observed MW: 75-90 kDa

Purity: ＞90% by SDS-PAGE

Endotoxin: <0.1EU/μg

Conjugation: Unconjugated

• Tag

  with C-His tag

• Physical Appearance

  Lyophilized powder

• Storage Buffer

  Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

• Reconstitution

  Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

• Stability & Storage

  12 months from date of receipt, -20 to -70 °C as supplied.
  6 months, -20 to -70 °C under sterile conditions after reconstitution.
  1 week, 2 to 8 °C under sterile conditions after reconstitution.
  Please avoid repeated freeze-thaw cycles.

The tau proteins (abbreviated from tubulin associated unit) form a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). They have roles primarily in maintaining the stability of microtubules in axons and are abundant in the neurons of the central nervous system (CNS), where the cerebral cortex has the highest abundance. They are less common elsewhere but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. Pathologies and dementias of the nervous system such as Alzheimer’s disease and Parkinson’s disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles.